陈实（Chen Shi）
职称：美国微生物科学院Fellow 国家基金委杰出青年基金 科技部中青年科技创新领军人才 科技部重点专项首席科学家 楚天学者特聘教授 博士生导师
通讯地址: 湖北省武汉市武昌区东湖路185号（邮编：430071），9778818威尼斯
办公地址:9778818威尼斯医学部(中南医院旁边)8号楼801室
学习工作简历
1994 – 2000 华中农业大学生物技术六年制本硕连读专业
2000 – 2004 获上海交通大学生物化学与分子生物学博士学位
2001 – 2002 作为访问博士生赴韩国高等科技学院合作研究
2005 – 2007 赴美国麻省理工学院进行博士后研究
2007 – 2011 在美国哈佛大学进行研究工作
2011 - 至今 9778818威尼斯教授
业绩概要
以通讯或共同通讯作者在Nature Microbiology, Nature Chemical Biology, Nature Catalysis，PNAS (3篇), Nature Communications (4篇), Advanced Materials(2篇)、Advanced Science (4篇), Advanced Functional Materials, Cell Death & Differentiation, Angew Chem Int Ed, Autophagy, Nucleic Acids Research, Annual Review of Microbiology, Clinical Microbiology Reviews, FEMS Microbiology Reviews, Trends in Cell Biology, Medicinal Research Reviews, Biotechnology Advances, Molecular Neurodegeneration, Theranostics (2篇)，SCIENCE CHINA Chemistry, Protein and Cell，Cell Death & Disease，mBio (4篇), Analytical Chemistry等发表论文。
入选美国微生物科学院院士，主持科技部重点专项、国家基金委杰出青年基金、重点国际合作项目、面上项目等。致力于微生物学、化学生物学、精准医学、基因组修饰与编辑、合成生物学与药物发现方面的研究。
任国际化学生物学协会国际委员会成员，曾任9778818威尼斯院长、中国遗传学会微生物遗传专业委员会副主任委员（2018-2023）、Microbiological Research（2017-2020）和BMC Systems Biology（2018-2020）副主编，现任mLife、Engineering Microbiology等编委。
获奖情况
2020年 美国微生物科学院Fellow (Fellow of American Academy of Microbiology)
2019年 国家基金委杰出青年基金
2018年 科技部中青年科技创新领军人才
2018年 9778818威尼斯朱裕璧医学奖
2017年 湖北省医学领军人才
2017年 9778818威尼斯杰出青年（教职工）
2016年 中国微生物学会学术年会优秀学术论文奖
2015年 高等学校科学研究优秀成果奖自然科学一等奖
2015年 9778818威尼斯医学部医学突出贡献奖
2014年 中国微生物学会学术年会优秀学术论文奖
2012年 9778818威尼斯医学部医学突出贡献奖
2012年 国家青年特聘专家
2012年 湖北省楚天学者特聘教授
2008年 国家自然科学奖二等奖
2008年 新加坡陈嘉庚基金会第六届陈嘉庚青少年发明奖（上海）（铜奖）
2007年 《环球科学》2007年度全球十大科学新闻
2007年 上海市优秀发明奖选拔赛二等奖
2007年 新加坡陈嘉庚基金会第五届陈嘉庚青少年发明奖（上海）（铜奖）
2006年 上海市研究生学位论文优秀成果
2004年 上海市科技进步一等奖
2004年 上海市新长征突击手“标兵”
2004年 新加坡陈嘉庚基金会第二届陈嘉庚青少年发明奖（上海）（银奖）
2004年 明治乳业生命科学优秀奖
2004年 上海-联合利华研究与发展基金奖学金
研究兴趣与方向
1．基因组修饰编辑与合成生物学
利用最新发展的合成生物学前沿技术，对细胞或病原菌基因组进行精确地鉴定、修饰和编辑，鉴定宿主对病毒或病原菌的防御机制，结合表观遗传学、基因组学、蛋白组学等，发展出特定设计的工程细胞，解析这些表观遗传学修饰或基因组改造对细胞生命活动或对病原菌侵染能力的影响，并阐明这些修饰或改造对超级耐药菌的作用。
2．创新药物的设计与改造
依据药物合成的机理及对病毒、神经、肿瘤等重大疾病致病机理的认识，通过全面的病理分析，合理筛选并设计潜在药物，并通过前沿技术合成生物学、基因组编辑、基因工程、蛋白质工程、代谢工程及组合生物合成途径进行优化改造，发展相应的创新药物、mRNA疫苗和治疗手段，以获得性能改善并可应用于临床的创新药物。
代表性学术论文: (*corresponding author) (#co-first author)
1. Susu Jiang#, Chao Chen#, Wanqiu Huang, Yue He, Xuan Du, Yi Wang, Hongda Ou, Zixin Deng, Congrui Xu, Lixu Jiang, Lianrong Wang, Shi Chen*. A widespread phage-encoded kinase enables evasion of multiple host antiphage defense systems. Nature Microbiology, 2024, DOI: 10.1038/s41564-024-01851-2.
2. Tianchen An#, Qian Tan#, Lixu Jiang, Li Liua, Xing Jiang, Liying Liu, Xiaofei Chang, Xihao Tian, Zixin Deng, Shuai Gao, Lianrong Wang*, Shi Chen *. An unusual DNA backbone sulfur modification pathway via an adenylated intermediate modulates the Tdp antiphage defense machinery in thermophiles. Nature Chemical Biology, 2024. Accepted.
3. Dan Wu, Yaqian Tang, Siwei Chen, Yue He, Xiaofei Chang, Wenzhong Zheng, Zixin Deng, Zhiqiang Li, Lianrong Wang*, Geng Wu*, Shi Chen*. The functional coupling between restriction and DNA phosphorothioate modification systems underlying the DndFGH restriction complex. Nature Catalysis. 2022, December, 5, 1131–1144.
4. Chao Chen#, Lianrong Wang#, Si Chen, Xiaolin Wu, Meijia Gu, Xi Chen, Susu Jiang, Yunfu Wang Zixin Deng, Peter C. Dedon, Shi Chen*. Convergence of DNA methylation and phosphorothioation epigenetics in bacterial genomes. Proc Natl Acad Sci U S A, 2017, 114(17): 4501–4506. doi: 10.1073/pnas.1702450114.
5. Lei Xiong#, Siyi Liu#, Si Chen#, Yao Xiao#, Bochen Zhu, Yali Gao, Yujing Zhang, Beibei Chen, Jie Luo, Zixin Deng, Xiangdong Chen, Lianrong Wang, Shi Chen*. A new type of DNA phosphorothioation-based antiviral 1 system in archaea. Nature Communications, 2019, 10:1688 | https://doi.org/10.1038/s41467-019-09390-9.
6. Tong Tong#, Si Chen#, Lianrong Wang#, You Tang#, Jae Yong Ryu, Susu Jiang, Xiaolin Wu, Chao Chen, Jie Luo, Zixin Deng, Zhiqiang Li, Sang Yup Lee*, Shi Chen*. Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria, Proc Natl Acad Sci U S A, 2018,115(13):2988-2996. doi:10.1073/pnas.1721916115.
7. Haiyan Gao#, Xinqi Gong#, Jinchuan Zhou, Yubing Zhang, Jinsong Duan, Yue Wei, Liuqing Chen, Zixin Deng, Jiawei Wang, Shi Chen*, Geng Wu*, Lianrong Wang*. Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE. Nature Communications, 2022, November, 13:6773.
8. Yajie Wang, Yuting Yang, Ying Cai, Ayikaimaier Aobulikasimu, Yuexin Wang, Chuanwei Hu, Zhikang Miao, Yue Shao, Mengna Zhao, Yue Hu, Chang Xu, Xinjun Chen，Zhiqiang Li, Jincao Chen, Lianrong Wang*, Shi Chen*. Endo‐Lysosomal Network Disorder Reprograms Energy Metabolism in SorL1‐Null Rat Hippocampus. Advanced Science, 2024, doi.org/10.1002/advs.202407709.
9. Luojia Chen#, Ming-Yu Wu#,*, Si-Ling Chen#, Rui Hu, Yifei Wang, Weijuan Zeng, Shun Feng, Min Ke*, Lianrong Wang*, Shi Chen*, Meijia Gu*. The Guardian of Vision: Intelligent Bacteriophage-Based Eyedrops for Clinical Multidrug-Resistant Ocular Surface Infections. Advanced Materials, 2024, doi.org/10.1002/adma.202407268.
10. Lianrong Wang*, Yaqian Tang, Zixin Deng, Shi Chen*. DNA Phosphorothioate Modification Systems and Associated Phage Defense Systems. Annual Review of Microbiology, 2024, DOI.org/10.1146/annurev-micro-041222-014330.
11. Pengqing Nie, Cheng Zhang, Fengyi Wu, Shi Chen*, Lianrong Wang*. The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability. Advanced Science, 2024, DOI: 10.1002/advs.202307751.
12. Yachen Wu#, Wenzhong Zheng, Guofeng Xu, Lijun Zhu, Zhiqiang Li, Jincao Chen, Lianrong Wang*, Shi Chen*. C9orf72 controls hepatic lipid metabolism by regulating SREBP1 transport. Cell Death & Differentiation, 2024, DOI.org/10.1038/s41418-024-01312-7.
13. Ming-Yu Wu#, Luojia Chen#, Qingrong Chen, Rui Hu, Xiaoyu Xu, Yifei Wang, Jie Li, Shun Feng, Changjiang Dong, Xiao-Lian Zhang, Zhiqiang Li, Lianrong Wang*, Shi Chen*, Meijia Gu*. Engineered Phage with Aggregation-Induced Emission Photosensitizer in Cocktail Therapy against Sepsis. Advanced Materials. 2023, 35, 2208578, DOI: 10.1002/adma.202208578.
14. Xuan Zou, Xiaohong Xiao, Ziran Mo, Yashi Ge, Xing Jiang, Ruolin Huang, Mengxue Li, Zixin Deng, Shi Chen*, Lianrong Wang*, Sang Yup Lee*. Systematic strategies for developing phage resistant Escherichia coli strains. Nature Communications, 2022, 13: 4491.
15. Ming-Yu Wu#, Xiaoyu Xu#, Rui Hu#, Qingrong Chen, Luojia Chen, Yuncong Yuan, Jie Li, Li Zhou, Shun Feng, Lianrong Wang*, Shi Chen*, Meijia Gu*. A membrane-targeted photosensitizer prevents drug resistance and induces immune response in treating Candidiasis. Advanced Science. 2023; e2207736. DOI: 10.1002/advs.202207736.
16. Bo Cao#, Chao Chen#, Michael S. DeMott#, Qiuxiang Cheng, Tyson A. Clark, Xiaolin Xiong, Xiaoqing Zheng, Vincent Butty, Stuart S. Levine, George Yuan, Matthew Boitano, Khai Luong, Yi Song, Xiufen Zhou, Zixin Deng, Stephen W. Turner, Jonas Korlach, Delin You*, Lianrong Wang*, Shi Chen*, Peter C. Dedon. Genomic mapping of phosphorothioates reveals partial modification of short consensus sequences. Nature Communications, 2014, 5, doi:10.1038/ncomms4951.
17. Lianrong Wang, Susu Jiang, Chao Chen, Wei He, Xiaolin Wu, Fei Wang, Tong Tong, Xuan Zou, Zhiqiang Li, Jie Luo, Zixin Deng, Shi Chen*. Synthetic genomes: from DNA synthesis to genome design. Angew Chem Int Ed., 2018, 57: 2~11.
18. Meijia Gu#, Xiaoyu Xu#, Ming-Yu Wu#, Chao Chen, Yuncong Yuan, Qingrong Chen, Yidan Sun, Luojia Chen, Chao Shen, Peng Guo, Shujie Liu, Engui Zhao*, Shi Chen*, Sijie Chen*. Simultaneous photodynamic eradication of tooth biofilm and tooth whitening with an aggregation-induced emission luminogen. Advanced Science, 2022, 9, 2106071.
19. Yanlian Chen, Zhiqiang Deng, Shuai Jiang, Qian Hu, Haiying Liu, Zhou Songyang, Wenbin Ma, Shi Chen*, Yong Zhao*. Human cells lacking coilin and Cajal bodies areproficient in telomerase assembly, trafficking and telomere maintenance. Nucleic Acids Research, 2014, 1-11, doi: 10.1093/nar/gku1277.
20. Lianrong Wang, Shi Chen*, Kevin L. Vergin, Stephen J. Giovannoni, Simon W. Chan, Michael S. DeMott, Koli Taghizadehe, Otto X. Cordero, Michael Cutler, Sonia Timberlake, Eric J. Alm, Martin F. Polz, Jarone Pinhassi, Zixin Deng, Peter C. Dedon*. DNA phosphorothioation is widespread and quantized in bacterial genomes. Proc Natl Acad Sci U S A, 2011, 108 (7): 2963-2968.
21. Wenzhong Zheng#, Kexin Wang#, Yachen Wu#, Ge Yan, Chi Zhang, Zhiqiang Li, Lianrong Wang*, Shi Chen*. C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α. Theranostics, 2022, 12, Issue 17.
22. Zhiqiang Deng, Junghyun Lim, Qian Wang, Kerry Purtell, Shuai Wu, Gloria M. Palomo, Haiyan Tan, Giovanni Manfredi, Yanxiang Zhao, Junmin Peng, Bo Hu, Shi Chen*, Zhenyu Yue*. ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/ NRF2 anti-oxidative stress pathway. Autophagy, 2019. DOI: 10.1080/15548627.2019.1644076.
23. Yajie Wang#, Zhikang Miao# , Chang Xu, Ying Cai, Yuting Yang, Yue Hu, Mengna Zhao, Yue Shao, Zhiqiang Li, Jincao Chen, Shi Chen*, Lianrong Wang*. Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats. Cell Death & Disease, 2023 May 13;14(5):325. DOI: 10.1038/s41419-023-05846-5.
24. Huang Q, Wang F, Yuan C, He Z, Rao L, Cai B, Chen B, Jiang S, Li Z, Chen J, Liu W, Guo F, Ao Z*, Shi Chen*, Zhao X*. Gelatin nanoparticle-coated silicon beads for density-selective capture and release of heterogeneous circulating tumor cells with high purity. Theranostics, 2018, 8(6): 1624~1635.
25. Deng Z, Purtell K Lachance V, Wold M, Shi Chen*, Yue Z*. Autophagy Receptors and Neurodegenerative Diseases. Trends in Cell Biology, 2017, 27(7).491~504. doi.org/10.1016/j.tcb.2017.01.001.
26. Fei Wang#, Lianrong Wang#, Xuan Zou, Suling Duan, Zhiqiang Li, Zixin Deng, Jie Luo, Sang Yup Lee*, Shi Chen*. Advances in CRISPR-Cas systems for RNA targeting, tracking and editing. Biotechnology Advances. 2019 Mar 27. pii: S0734-9750(19)30052-7. doi: 10.1016/j.biotechadv.2019.03.016.
27. Lianrong Wang, Susu Jiang, Zixin Deng, Peter Dedon, Shi Chen*. DNA phosphorothioate modification–a new multi-functional epigenetic system in bacteria. FEMS Microbiology Reviews. 2019, 43(2), 109–122. https://doi.org/10.1093/femsre/fuy036.
28. Meijia Gu#*, Luojia Chen#, Rui Hu, Qingrong Chen, Jianbo Liu*, Lianrong Wang*, Shi Chen*. Aggregation-induced emission: recent applications in infectious diseases. SCIENCE CHINA Chemistry, 2023 Oct 24. doi.org/10.1007/s11426-023-1825-7.
29. Zhiqiang Deng, Patricia Sheehan, Shi Chen*, Zhenyu Yue*. Is amyotrophic lateral sclerosis/frontotemporal dementia an autophagy disease? Molecular Neurodegeneration (2017) 12:90. DOI 10.1186/s13024-017-0232-6.
30. Xuan Zou#, Lianrong Wang#, Zhiqiang Li, Jie Luo, Yunfu Wang, Zixin Deng, Shiming Du, Shi Chen*. Genome engineering and modification toward synthetic biology for the production of antibiotics. Medicinal Research Reviews, 2018, 38(1): 229~260.
31. Shi Chen*, Lianrong Wang, Zixin Deng*. Twenty years hunting for sulfur in DNA. Protein & Cell, 2010, 1(1): 14-21.
