王隆飞 

职务/职称：教授、博士生导师 

教育经历：

09/2001 –07/2005 华中农业大学，生物技术学士学位

09/2005 – 07/2011 中国科学院生物物理研究所，生物化学与分子生物学博士学位

研究经历：

07/2011 – 09/2011 中国科学院生物物理研究所，助理研究员

10/2011 – 04/2017 哈佛医学院，博士后

05/2017 – 07/2021 哈佛医学院波士顿儿童医院，讲师

研究方向：

研究领域：分子免疫学，结构药理学

研究兴趣：

    1.阐释宿主细胞防御病原体的分子机制

    2.研究免疫细胞表面受体的信号传导的结构基础

    3.研发可用于调控免疫信号通路的小分子和生物大分子（抗体，肽段，核酸等） 

学术论文（*共同第一作者，#共同通讯作者）：

1. Vora, S.M.*, Fontana, P.*, Mao, T., Leger, V., Zhang, Y., Fu, T.M., Lieberman, J., Gehrke, L., Shi, M.^#, Wang, L.^#, Iwasaki, A., Wu, H.^#, (in press). Targeting Stem-loop 1 of the SARS-CoV-2 5’UTR to suppress viral translation and Nsp1 evasion. Proceedings of the National Academy of Sciences.

2. Huang, F., Lu, X., Yu, C., Sliz, P., Wang, L.^#, Zhu, B.^#, 2021. Molecular dissection of the primase and polymerase activities of deep‐sea phage NrS‐1 primase-polymerase. Frontiers in Microbiology, 3887.

3. Wang, L.*, Sharif, H.*, Vora, S.M., Zheng, Y., Wu, H., 2021. Structures and functions of the inflammasome engine. The Journal of Allergy and Clinical Immunology, 147(6).

4. Xia, S., Zhang, Z., Magupalli, V.G., Pablo, J.L., Dong, Y., Vora, S.M., Wang, L., Fu, T.M., Jacobson, M.P., Greka, A., Lieberman, J., Ruan, J., Wu, H., 2021. Gasdermin D pore structure reveals preferential release of mature interleukin-1. Nature, 21, 1-5.

5. Wang, L.^#, Wu, D., Robinson, V.C., Wu, H. ^#, Fu, T.-M.^#, 2020. Structures of a complete human V-ATPase reveal mechanisms of its assembly Molecular Cell, 80, 1-11.

6. Yu, C.*, Wang, L.*, Rowe, R.G., Han, A., Jia, W., McMahon, C., Baier, A.S., Huang, Y.C., Marion, W., Pearson, D.S., Kruse, A.C., Daley, G.Q., Wu, H., Sliz, P., 2020. A nanobody targeting the LIN28:let-7 interaction fragment of TUT4 blocks uridylation of let-7. Proceedings of the National Academy of Sciences, 117 (9) 4653-4663.

7. Gu, L.*, Wang, L.*, Chen, H.*, Hong, J.*, Shen, Z., Dhall, A., Lao, T., Liu, C., Wang, Z., Xu, Y., Tang, H.-W., Chakraborty, D., Chen, J., Liu, Z., Rogulja, D., Perrimon, N., Wu, H., Shi, Y., 2020. CG14906 (mettl4) mediates m 6 A methylation of U2 snRNA in Drosophila. Cell Discovery, 6 (1), 1-4.

8. Chen, H., Gu, L., Orellana, E.A., Wang, Y., Guo, J., Liu, Q., Wang, L., Shen, Z., Wu, H., Gregory, R.I., Xing, Y., Shi, Y., 2020. METTL4 is an snRNA m6Am methyltransferase that regulates RNA splicing. Cell Research, [Epub ahead of print].

9. Wang, L.*, Negro, R.*, Wu, H., 2020. TRPM2, linking oxidative stress and Ca2+ permeation to NLRP3 inflammasome activation. Current Opinion in Immunology, 62:131-135. 

10. Wang, L., Wu, H., 2019. Keeping the Death Protein in Check. Immunity, 51: 1-2.

11. Xia, S.*, Wang, L.*, Fu, T.M., Wu, H., 2019. Mechanism of TRPM2 channel gating revealed by cryo-EM. FEBS Journal, 286: 3333-3339.

12. Wang, L.*, Fu, T. M.*, Zhou, Y., Xia, S., Greka, A., and Wu, H., 2018. Structures and gating mechanism of human TRPM2. Science, 362: 1377 (eaav4809).

13. Wang, L.*, Rowe, R.G.*, Jaimes, A., Yu, C., Nam, Y., Pearson, D.S., Zhang, J., Xie, X., Marion, W., Heffron, G.J., Daley, G.Q., and Sliz, P., 2018. Small-Molecule Inhibitors Disrupt let-7 Oligouridylation and Release the Selective Blockade of let-7 Processing by LIN28. Cell Reports, 23: 3091-3101. 

14. Wang, L.^#, Yang, Q., Jaimes, A., Wang, T., Strobelt, H., Chen, J., and Sliz, P.^#, 2018. MightyScreen: an open-source visualization application for screening data analysis. SLAS DISCOVERY, 23: 218-223.

15. Choe, J., Lin, S., Zhang, W., Liu, Q., Wang, L., Ramirez-Moya, J., Du, P., Kim, W., Tang, S., Sliz, P. and Santisteban, P., George, R.E., Richard, W.G., Wong, K.K., Locker, N., Slack, F.J., Gregory, R.I., 2018. mRNA circularization by METTL3–eIF3h enhances translation and promotes oncogenesis. Nature, 561: 556-560.

16. Wang, L.*, Nam, Y.*, Lee, A.K., Yu, C., Roth, K., Chen, C., Ransey, E.M. and Sliz, P., 2017. LIN28 zinc knuckle domain is required and sufficient to induce let-7 oligouridylation. Cell Reports, 18: 2664-2675.

17. Zhu, B., Wang, L., Mitsunobu, H., Lu, X., Hernandez, A.J., Yoshida-Takashima, Y., Nunoura, T., Tabor, S. and Richardson, C.C., 2017. Deep-sea vent phage DNA polymerase specifically initiates DNA synthesis in the absence of primers. Proceedings of the National Academy of Sciences, 114: E2310-E2318. 

18. Ou, X., Guo, J., Wang, L., Yang, H., Liu, X., Sun, J. and Liu, Z., 2017. Ion-and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel. BMC Biology, 15: 31. 

19. Zhang, J., Ratanasirintrawoot, S., Chandrasekaran, S., Wu, Z., Ficarro, S.B., Yu, C., Ross, C.A., Cacchiarelli, D., Xia, Q., Seligson, M., Shinoda, G., Xie, W., Cahan, P., Wang, L., Ng, S.C., Tintara, S., Trapnell, C., Onder, T., Loh, Y.H., Mikkelsen, T., Sliz, P., Teitell, M.A., Asara, J.M., Marto, J.A., Li, H., Collins, J.J., and Daley, G.Q., 2016. LIN28 regulates stem cell metabolism and conversion to primed pluripotency. Cell Stem Cell, 19: 66-80. 

20. Du, P., Wang, L., Sliz, P. and Gregory, R.I., 2015. A biogenesis step upstream of microprocessor controls miR-17~ 92 expression. Cell, 162: 885-899.

21. Wang, L., Zhao, F., Li, M., Zhang, H., Gao, Y., Cao, P., Pan, X., Wang, Z. and Chang, W., 2011. Conformational changes of rBTI from buckwheat upon binding to trypsin: implications for the role of the P8’ residue in the potato inhibitor I family. PLOS One, 6: e20950.

22. Pan, X., Li, M., Wan, T., Wang, L., Jia, C., Hou, Z., Zhao, X., Zhang, J. and Chang, W., 2011. Structural insights into energy regulation of light-harvesting complex CP29 from spinach. Nature Structural & Molecular Biology, 18: 309.

国际专利：

 Small-molecule Inhibitors of the Lin28/Let-7 Complex, US Patent Application No. 62/332,512.